The EST data can be searched and retrieved using the following
search criteria: *
EST Annotator:
List all EST Information, Annotation, Pathway and Go Term. And search EST
libraries
by multiple keywords at the same time.
This function performs a text search among the pre-computed BLAST results
against NR for all assembly
products, with an
E-value cutoff of 1e-5. Type in the name of the gene you are
interested in, keeping in mind that the description of NCBI accessions
references genes in different ways. For example, searching for “Annotation:
protein, Species: Oryza” will yield all BLAST results as
the following figure
1-a, figure 1-b, figure 1-c.
figure 1-a. Select Library Name and Click ’View/Search’ button, will list all
EST information.
figure 1-b. Search EST libraries by multiple keywords at the same time.
(Keyword: Annotation: protein
Species: Oryza)
figure 1-c. Snapshots of search results
TC (tentative contig) Annotator: List all TC
Information, Annotation, Pathway and Go Term. And search EST libraries by
multiple keywords at the same time, shows as the figure 2 below.
figure 2. Search EST libraries by
multiple keywords at the same time.
Gene Ontology:
Classification of TCs by GO vocabularies, shows as the figure 3 below.
figure 3. Snapshots of search
results
Metabolic Pathways(Tree view): Association
of TCs with metabolic and signaling pathways, shows as the figure 4 below.
figure 4 Snapshots of analytic
results
Metabolic Pathways(List view): Association of TCs
with metabolic and signaling pathways, shows as the figure 5-a, figure 5-b
below.
figure 5-a Snapshots of search
results
figure 5-b Snapshots of search
results
Search by BLAST (Phalaenopsis Transcriptome)
Search by BLAST (Orchidaceae Floral Transcriptome)
The user can paste sequence to BLAST against EST data sets from the
orchids. Alignment results could be viewed and the hit sequence could be
retrieved.
figure 6 Snapshots of BLAST
results
Multiple Gene
Network Analysis(Phalaenopsis Transcriptome)
figure 7 Gene Network Search
Multiple
Gene Network Analysis(Phalaenopsis Transcriptome)
figure 8 Multiple Gene Network Analysis